Fig. 3
The sensitivity of real-time PCR. 1–4 appropriate dilutions (102–105 copy DNA/μl) adenovirus strain/species 2/D isolated from infected CEK by FAdV adenovirus strain. ΔRn is an increment of fluorescent signal during successive cycles. a, b, c The derivative reporter value is plotted as the y-axis while the temperature is plotted as the x-axis. The melting temperature peak is 73.74 °C (b). The standard curve derived during amplification has been obtained and presented in (c)