Fig. 6

(a) Density of IBA1 staining (macrophages) normalized to baseline values at weeks 1 and 4 by treatment group in the Inner Wound, Wound Periphery, and Wound Center regions. Data are presented as median and range. (b) Representative photomicrograph of macrophages in tissue at week 4 in the Wound Periphery region of wounds treated with ECFC/MS. (c) Representative photomicrograph of macrophages in tissue at week 4 in the Wound Periphery region of wounds treated with MS. (d) Density of elastase staining (activated neutrophils) normalized to baseline values at weeks 1 and 4 by treatment group in the Inner Wound, Wound Periphery, and Wound Center regions. (e) Representative photomicrograph of neutrophils in tissue at week 4 in the Wound Center region of wounds treated with ECFC (f) Representative photomicrograph of neutrophils in tissue at week 4 in the Wound Center region of wounds treated with MS. Note that there is a subjectively greater density of macrophages in the Wound Periphery region of wounds at week 4 treated with MS (c) compared to wounds treated with ECFC/MS (b); there is also a subjectively greater density of neutrophils in the Wound Center region of wounds at week 4 treated with MS (f) compared to wounds treated with ECFCs (e). * indicates a significant difference between treatment groups, with MS alone wounds having the highest density of macrophages and neutrophils at week 4 (P < 0.05). Scale bars are 100 μm