Fig. 2

Schematic of wound biopsies and regions analyzed for quantification of immunohistochemical staining. (a) 2 wounds (blue squares) per horse were treated identically, but biopsied (orange circles) differently. 2 punch biopsies (one punch biopsy was fixed in formalin and the other was frozen) were collected at the leading edge of the wound once weekly, at the leading edge once 4 weeks after wound creation, or at the center of the wound once 4 weeks after wound creation. (b,c) Punch biopsies from wounds collected at either the center of the wound or the leading edge of showing the regions of interest (outlined in yellow) analyzed with the quantification software program. Biopsies are stained for collagen (blue) with Masson’s trichrome. “Inner Wound” (non-healed wound within advancing line of epithelialization); “Wound Periphery” (skin with epithelial cell cover, just on the periphery of the advancing line of epithelialization); and “Wound Center” (area at the center of the non-healed wound)