Table 2 Primer sequences of RT-PCR
Target | Primer sequence(5′-3′) | Accession no. | Product size (bp) |
|---|---|---|---|
claudin-1 | F:TCCAAGCTCACCAAAGAGGG | NM_001013611.2 | 128 |
R:ACCGGTGACAGACTGGTTTC | |||
TLR2 | F:TACAGATGCTACTGTGCCTGA | NM_001161650.1 | 102 |
R:CACTTTCCAGTGCCCAAGAG | |||
TLR4 | F:TTCCATGGCTTAACGTCGCT | NM_001030693.1 | 82 |
R:AGTGTCCGATGGGTAGGTCA | |||
Occludin | F:TGTGTAAGGCCCACACCTCT | NM_205128.1 | 92 |
R:TGCTCAGGGTACCATTCTGG | |||
TNF-α | F:GAGCGTTGACTTGGCTGTC | XM_015294124.2 | 64 |
R:GAGCGTTGACTTGGCTGTC | |||
IL-1β | F:ACTGGGCATCAAGGGCTA | XM_015297469.1 | 131 |
R:GGTAGAAGATGAAGCGGGTC | |||
MUC2 | F:TTCATGATGCCTGCTCTTGTC | XM_421035 | 93 |
R:CCTGAGCCTTGGTACATTCTTGT | |||
β-actin | F:TTGTCCACCGCAAATGCTTC | NM_205518.1 | 106 |
R:AGCCATGCCAATCTCGTCTT |