Effects of Lactobacillus plantarum on intestinal integrity and immune responses of egg-laying chickens infected with Clostridium perfringens under the free-range or the specific pathogen free environment

Table 10 Effects of LP and environment on inflammatory factors of ileum mucosa of chickens after CP administration

	IL-1β			TNF-α			TLR2			TLR4
	1st	3rd	10th	1st	3rd	10th	1st	3rd	10th	1st	3rd	10th
SPFC	1.00	1.00	1.00	1.00	1.00	2.21	1.00	1.00	1.00	1.00	1.00	1.00
SPFL	0.60	0.69	0.55	0.80	0.69	0.73	0.52	0.45	0.62	0.68	0.46	0.54
FRC	0.67	1.72	1.25	1.71	1.15	1.00	0.83	0.59	1.90	0.83	1.00	1.51
FRL	0.53	1.02	0.73	0.81	0.48	0.79	0.61	0.38	0.60	0.71	0.31	0.58
SEM	0.147	0.140	0.108	0.122	0.093	0.132	0.141	0.197	0.258	0.130	0.204	0.147
Main effects
Environment
SPF	0.80	0.88	0.83	0.93	0.86	1.47	0.79	0.75	0.86	0.86	0.80	0.83
FRS	0.61	1.32	0.99	1.19	0.86	0.92	0.72	0.51	1.25	0.78	0.65	1.04
Lactobacillus
NLP	0.82	1.27	1.09	1.27	1.07	1.45	0.93	0.79	1.34	0.92	1.00	1.19
LP	0.56	0.88	0.64	0.81	0.60	0.76	0.56	0.42	0.61	0.69	0.38	0.56
P-value
Environment	0.510	0.086	0.349	0.171	0.860	0.055	0.880	0.552	0.416	0.795	0.859	0.372
Lactobacillus	0.372	0.099	0.051	0.046	0.022	0.010	0.235	0.352	0.135	0.404	0.163	0.039
Environment × Lactobacillus	0.667	0.505	0.873	0.176	0.347	0.037	0.664	0.667	0.398	0.709	0.865	0.438

ISSN: 1746-6148