Skip to main content

Table 1 IC50 values (μmol/L) of betulinic acid for primary equine cells determined by CVS and MTS assay

From: Betulinic acid shows anticancer activity against equine melanoma cells and permeates isolated equine skin in vitro

cells24 h48 h96 h
MTS assay
 eRGO122.8 (−3–48)20.7 (13–29)12.7 (11–15)
 MelDuWi34.6 (24–45)31.7 (25–38)23.6 (13–34)
 PriFi120.4 (19–22)18.0 (17–19)13.8 (7–21)
 PriFi224.8 (11–39)22.7 (1–49)13.3 (11–16)
Crystal violet staining assay
 eRGO125.9 (20–32)21.2 (− 2–44)19.6 (11–29)
 MelDuWi49.2 (31–67)35.8 (− 22–94)21.6 (5–38)
 PriFi158.0 (52–64)52.2 (39–65)14.5 (14–15)
 PriFi230.3 (17–44)29.1 (6–53)13.8 (10–18)
  1. Cytotoxic (MTS assay) and antiproliferative (crystal violet staining assay) effects of betulinic acid on primary equine melanoma cells (eRGO1 and MelDuWi) and primary equine dermal fibroblasts (PriFi1 and PriFi2) after a treatment duration of 24, 48, or 96 h. Data represent mean IC50 values (μmol/L) of 6–8 independent experiments with 95% confidence interval in parentheses