Table 1 IC50 values (μmol/L) of betulinic acid for primary equine cells determined by CVS and MTS assay
cells | 24 h | 48 h | 96 h |
|---|---|---|---|
MTS assay | |||
eRGO1 | 22.8 (−3–48) | 20.7 (13–29) | 12.7 (11–15) |
MelDuWi | 34.6 (24–45) | 31.7 (25–38) | 23.6 (13–34) |
PriFi1 | 20.4 (19–22) | 18.0 (17–19) | 13.8 (7–21) |
PriFi2 | 24.8 (11–39) | 22.7 (1–49) | 13.3 (11–16) |
Crystal violet staining assay | |||
eRGO1 | 25.9 (20–32) | 21.2 (− 2–44) | 19.6 (11–29) |
MelDuWi | 49.2 (31–67) | 35.8 (− 22–94) | 21.6 (5–38) |
PriFi1 | 58.0 (52–64) | 52.2 (39–65) | 14.5 (14–15) |
PriFi2 | 30.3 (17–44) | 29.1 (6–53) | 13.8 (10–18) |