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Table 1 Determination of the optimal rHc-CS coating concentration and serum dilution for indirect-ELISA

From: Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat

Serum Dilution OD450 values of rHc-CS at increasing coating concentrations
0.070.140.280.561.122.254.5
1:25(P)0.460.791.211.201.361.561.85
(N)0.190.300.350.530.640.941.61
P/N2.452.673.412.272.131.661.15
1:50(P)0.400.681.191.181.211.491.63
(N)0.180.210.290.340.550.771.41
P/N2.203.214.053.452.211.941.15
1:100(P)0.350.511.011.201.281.381.40
(N)0.170.170.200.260.440.681.29
P/N2.072.885.084.572.912.011.08
1:200(P)0.230.410.780.860.920.971.22
(N)0.170.130.160.220.300.501.00
P/N1.363.084.693.823.021.921.22
  1. Note: Bold represent the optimum conditions for this indirect-ELISA method, the highest P/N value is 5.08
  2. Abbreviations: P Positive serum, N Negative serum