Recombinant cold shock domain containing protein is a potential antigen to detect specific antibody during early and late infections of Haemonchus contortus in goat

Table 1 Determination of the optimal rHc-CS coating concentration and serum dilution for indirect-ELISA

Serum Dilution		OD₄₅₀ values of rHc-CS at increasing coating concentrations
Serum Dilution		0.07	0.14	0.28	0.56	1.12	2.25	4.5
1:25	(P)	0.46	0.79	1.21	1.20	1.36	1.56	1.85
	(N)	0.19	0.30	0.35	0.53	0.64	0.94	1.61
	P/N	2.45	2.67	3.41	2.27	2.13	1.66	1.15
1:50	(P)	0.40	0.68	1.19	1.18	1.21	1.49	1.63
	(N)	0.18	0.21	0.29	0.34	0.55	0.77	1.41
	P/N	2.20	3.21	4.05	3.45	2.21	1.94	1.15
1:100	(P)	0.35	0.51	1.01	1.20	1.28	1.38	1.40
	(N)	0.17	0.17	0.20	0.26	0.44	0.68	1.29
	P/N	2.07	2.88	5.08	4.57	2.91	2.01	1.08
1:200	(P)	0.23	0.41	0.78	0.86	0.92	0.97	1.22
	(N)	0.17	0.13	0.16	0.22	0.30	0.50	1.00
	P/N	1.36	3.08	4.69	3.82	3.02	1.92	1.22

Note: Bold represent the optimum conditions for this indirect-ELISA method, the highest P/N value is 5.08
Abbreviations: P Positive serum, N Negative serum

ISSN: 1746-6148