Incorporation of a truncated form of flagellin (TFlg) into porcine circovirus type 2 virus-like particles enhances immune responses in mice

Table 1 Primers used in this study

Genes	Name	Primer Sequence (5′-3′)
SUMO	SUMO-F	GCCATGGGTCATCACCATCATCATCACGTGGGTCGGACTCAGAAG
	SUMO-R	ACCTCCAATCTGTTCGCGGTGAGCCTCAAT
	Cap-F	GAACAGATTGGAGGTATGACCTACCCGCGTCGTCG
Cap	Cap-R1^a	CTCGAGTTACGGGTTCAGCGGAGGGTC
Cap	Cap-R2^b	CGGGTTCAGCGGAGGGTCCTTCA
TFlg	TFlg-F	ACCCTCCGCTGAACCCGATCAACAACAACCTGCA
TFlg	TFlg-R	GCCGCTCGAGTTAGGAGTCGAGGTCAGACTGG

^a Primer used to clone the SUMO-Cap gene; ^b Primer used to clone the SUMO-Cap-TFlg gene; The underlined sequences represent the restriction enzyme site (NcoI and XhoI). The sequences in lowercase letters indicate the His6 tag. The sequences in bold indicate the stop codon

ISSN: 1746-6148