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Fig. 6 | BMC Veterinary Research

Fig. 6

From: Heterogeneity of Moraxella isolates found in the nasal cavities of piglets

Fig. 6

Phagocytosis susceptibility of selected Moraxella spp. strains. Selected M. pluranimalium strains (gray bars), strains from the putative new species cluster (black bars) and M. porci strains (streaked bars). FITC-labelled Moraxella spp. strains were incubated with porcine alveolar macrophages (PAMs) during 1 h at 37 °C. After washing to eliminate unbound bacteria, association of bacteria with PAMs was measured by flow cytometry and expressed as % of fluorescent PAMs. H. parasuis susceptible strain SW114 and resistant strain Nagasaki were included as control for the assay (white bars) and SW114 levels of phagocytosis were used to normalize the results of different tests. In addition, strains UK1–20 and VL6–6 after 27 passages on agar plates were also tested (dotted bars). Results represent the average of duplicate wells from 2 different assays (error bars: standard deviation)

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