Table 1 IHC protocol for EGFR, Her-2, Her-3, Her-4, Ki67, Melan-A, Melanoma antigen antibodies
From: Diverse roles of epidermal growth factors receptors in oral and cutaneous canine melanomas
Antibody | Manufacturer | Clone | Dilution | Incubation time (h) | Antigen retrieval | Amplification |
|---|---|---|---|---|---|---|
EGFR | Invitrogen | 31G7 | 1:50 | 16 h | Pepsina | Advance HRP |
Her-2 | Dako | Polyclonal | 1:200 | 16 h | Citrate buffer + WBb | Advance HRP |
Her-3 | Genetex | Polyclonal | 1:100 | 30 min | EDTA + WBb | Histofine |
Her-4 | Santa Cruz | C18 | 1:100 | 16 h | Citrate buffer + Pascalc | Histofine |
Ki-67 | Dako | MIB-1 | 1:50 | 16 h | Citrate buffer + Pascalc | Novolink |
Melan-A | Dako | A103 | 1:100 | 16 h | Citrate buffer + Pascalc | Novolink |
Melanoma Antigen | Santa Cruz | PNL-2 | 1:100 | 16 h | Citrate buffer + Pascalc | Novolink |