From: Molecular alterations of the TLR4-signaling cascade in canine epilepsy
Primary antibody | Dilution | Manufacturer | Secondary antibody | Dilution | Manufacturer |
---|---|---|---|---|---|
monoclonal mouse anti-Hsp70/72; C92F3A-5 | 1:100 | Enzo Life Sciences, Lörrach, Germany | Biotinylated goat-anti-mouse | 1:200 | Dianova / Jackson GmbH, Hamburg, Germany |
monoclonal mouse anti-TLR4; sc-293,072 | 1:500 | Santa Cruz Biotechnology, Heidelberg, Germany | Biotinylated goat-anti-mouse, Vector BA-9200 | 1:500 | BIOZOL Diagnostica Vertrieb GmbH, Eching Germany |
polyclonal rabbit anti-HMGB1; ab227168 | 1:100 | Abcam, Cambridge, UK | Biotinylated goat-anti-rabbit, 111–065-003 | 1:500 | Dianova / Jackson GmbH, Hamburg, Germany |
monoclonal mouse anti-NeuN; MAB377 | 1:100 | Millipore/ Merck Chemicals GmbH, Darmstadt, Germany | Biotinylated goat-anti-mouse | 1:500 | Vector Laboratories, Burlingame, California, USA |