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Table 7 Primary and secondary antibodies with dilution factor and manufacturing company

From: Molecular alterations of the TLR4-signaling cascade in canine epilepsy

Primary antibody

Dilution

Manufacturer

Secondary antibody

Dilution

Manufacturer

monoclonal mouse anti-Hsp70/72; C92F3A-5

1:100

Enzo Life Sciences, Lörrach, Germany

Biotinylated goat-anti-mouse

1:200

Dianova / Jackson GmbH, Hamburg, Germany

monoclonal mouse anti-TLR4; sc-293,072

1:500

Santa Cruz Biotechnology, Heidelberg, Germany

Biotinylated goat-anti-mouse, Vector BA-9200

1:500

BIOZOL Diagnostica Vertrieb GmbH, Eching Germany

polyclonal rabbit anti-HMGB1; ab227168

1:100

Abcam, Cambridge, UK

Biotinylated goat-anti-rabbit, 111–065-003

1:500

Dianova / Jackson GmbH, Hamburg, Germany

monoclonal mouse anti-NeuN; MAB377

1:100

Millipore/ Merck Chemicals GmbH, Darmstadt, Germany

Biotinylated goat-anti-mouse

1:500

Vector Laboratories, Burlingame, California, USA