Fig. 2
From: Molecular alterations of the TLR4-signaling cascade in canine epilepsy
HMGB1 expression in all investigated areas. Representative histological images of HMGB1-positive stained cells in the CA1 region of the hippocampus of dogs of patient control (a), experimental control (b), structural (c), and idiopathic group (d). The majority of immunopositive cells are intensely stained and have the morphology of microglia cells. Cells with the morphology of neurons have a less intense staining appearing in the cytoplasm. Correlation analysis of HMGB1 positive area with age (e). Impact of epilepsy type on HMGB1 expression (O.D. and positive labeled area) in CA1 (f, k), CA3 (g, l), hilus (h, m), dentate gyrus (DG; i, n), and piriform lobe (Pir; j, o). Impact of seizure activity on HMGB1 expression in CA1 (p, u), CA3 (q, v), hilus (r, w), dentate gyrus (DG; s, x), piriform lobe (Pir; t, y). All data are given as mean ± SEM; p < 0.05 was considered statistically significant (*). CTRpat: patient control dogs; CTRexp: experimental control dogs; Cluster: dogs with cluster seizures; Structural: dogs with structural epilepsy; SE: dogs with status epilepticus; Idiopathic: dogs with idiopathic epilepsy. Scale bar 10 μm