Fig. 1

Analysis of purified C-strain E2 protein and monoclonal antibody 6B211. a Purified C-strain E2 protein mainly exists in its native dimeric conformation. After purification steps, the purified insect cell expressed C-strain E2 protein were treated without (Native) or with β-mercaptoethanol (Reduced) and separated by SDS-PAGE in a Mini-Protean TGX Gel (Bio-Rad, CA, USA); b 6B211 only react with the native C-strain proteins. Purified E2 proteins (native or reduced) were loaded on Mini-Protean TGX Gel. The proteins were then transferred to PVDF membrane and the membrane were blocked and incubated with 6B211