Fig. 3

Effect of oral NAC administration on L. amazonensis infected mice on NGF and mast cell infiltration in paw skin. Representative western blot showing the effect of NAC (200 mg/kg) on NGF (A, A’). Levels of NGF presented in the densitometric analyses of protein bands were normalized for β-actin. The number of mast cell was increased in L. amazonensis infected mice (C) compared to the sham animals (B). Administration of NAC (200 mg/kg) (D) reduced mast cells infiltration in paw skin; the mast cells score was done by independent observer (E). Data are representative of at least three independent experiments and are expressed as mean ± SEM from N = 5 mice/group. A representative blot is shown and densitometric analysis is reported. All figures are representative of at least three independent experiments for all animals from each group. Values are means ± SEM ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. L. amazonensis infected mice