Fig. 2

a Graphical representation of the Tn559 structure containing the dfrK gene integrated in the chromosomal radC gene of the isolate Staphylococcus pseudintermedius C5347 (GenBank accession number MT252966) and nucleotide substitutions detected in the non-coding region downstream the dfrK gene compared to Staphylococcus aureus transposon Tn559 (GenBank accession number FN677369). The nucleotide positions are set based on the whole Tn559. Nucleotide insertions and substitutions are colored in yellow. b Amino acid sequence alignment of radC gene and resultant RadC of Staphylococcus pseudintermedius strain C2719 (GenBank accession number HF679552), where transposon Tn558 was integrated, that of S. pseudintermedius C5347 (GenBank accession number MT252966). The position of the primer pair employed is indicated in grey. Nucleotide substitutions are colored in yellow and amino acid substitutions in blue