Fig. 4
From: Identification of a universal antigen epitope of influenza A virus using peptide microarray
Western-blotting analysis of HA2 protein from different subtypes of influenza A viruses (IAVs). Lysates (amount of protein was 50 μg) of chicken embryo fibroblasts (CEF) infected with IAVs at MOI of 0.01 for 12 h–15 h were incubated with a primary antibody (immune serum of BSA-conjugated H5 14th peptide) and a monoclonal antibody (mAb) against β-actin. Bands were visualized using a chemiluminescence imaging analysis system after incubation with horse radish peroxidase (HRP)-labeled secondary antibodies. H5–01: A/Mallard/Huadong/S/2005; H5–02: A/Duck/Huadong/wx1205/2016; H5–03: A/Goose/Huadong/yz1111/2016; H7–01: A/Chicken/Jiangsu/W1–8/15; H7–02: A/Chicken/Huadong/JD/17; H9–01: A/Chicken/Shanghai/F/98; H9–02: A/Chicken/Taixing/10/2010