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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Identification of a universal antigen epitope of influenza A virus using peptide microarray

Fig. 2

The SNRs of the reaction between sera against different subtypes of IAVs and H5 14–1 (a), H5 14–2 (b), H5 14–3 (c), H5 14–4 (d) or H7–14 (e) peptides. Synthetic peptides were sampled onto iPDMS (modified silica gel film) to form chip, sera were diluted 1:50 (v/v) with serum dilution buffer for binding activity assay. Signal-to-noise ratio (SNR, SNR = (signal strength–background intensity)/background intensity) was determined by using GenePix Pro 6.0 software. The dotted line represents the SNR = 2. H5–01: serum against A/Mallard/Huadong/S/2005; H5–02: serum against A/Duck/Huadong/wx1205/2016; H5–03: serum against A/Goose/Huadong/yz1111/2016; H5–04: serum against 2.3.4.4d vaccine strain; H5–05: serum against 2.3.2.1d vaccine strain; H7–01: serum against A/Chicken/Jiangsu/W1–8/15; H7–02: serum against A/Chicken/Huadong/JD/17; H9–01: serum against A/Chicken/Shanghai/F/98; H9–02: serum against A/Chicken/Taixing/10/2010. Each reaction was repeated for 3 times, SNR values were expressed as means ± standard deviation. X axis, sera against different subtypes of IAVs; Y axis, SNR values

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