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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goats

Fig. 2

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis on the optimized expression of TgGRA8 in E. coli strain Rosetta (DE3), Coomassie blue stained. Lane M: protein molecular weight marker. Lane 1 to 3: pellet fractions of cells grown at 20 °C after induction with 1.0 mM IPTG (4,8 h and overnight). Lane 4 to 6: uninduced total cell lysate of E. coli strain Rosetta (DE3)-pET21a-TgGRA8 (4,8 h and overnight)

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