Fig. 2From: Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goatsSodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis on the optimized expression of TgGRA8 in E. coli strain Rosetta (DE3), Coomassie blue stained. Lane M: protein molecular weight marker. Lane 1 to 3: pellet fractions of cells grown at 20 °C after induction with 1.0 mM IPTG (4,8 h and overnight). Lane 4 to 6: uninduced total cell lysate of E. coli strain Rosetta (DE3)-pET21a-TgGRA8 (4,8 h and overnight)Back to article page