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Table 2 Newly designed primers to amplify the whole genome sequence of DCH

From: Molecular detection and characterisation of Domestic Cat Hepadnavirus (DCH) from blood and liver tissues of cats in Malaysia

Primer Sequence Base pair Annealing Temperature
DCH A F: 5’-TGGGCAACATTACCTCAGGTCC-3’ 1700 56℃
R: 5’-GGAACAAAAGAGAACGCACAGG-3’
DCH B F: 5’-GGTCTGACGCCCAGGTTATG-3’ 1700 56℃
R: 5’-ACCACGAGTCTGCACTCTGC-3’
DCH C F: 5’-CTCAGGTCTTTGCCCACTCA-3’ 1155 55℃
R: 5’-AGCTGACTCCTCCCAACAGT-3’
DCH D F: 5’-AACTAAGCATGAACTCCGCC-3’ 1173 55℃
R: 5’-TGGGCCAACAGGTGCAATTT-3’