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Table 1 Occurrence of Mycobacterium avium in chickens and birds in selected area of Peninsular Malaysia

From: Isolation of Mycobacterium avium and other nontuberculous mycobacteria in chickens and captive birds in peninsular Malaysia

Farm ID

Total samples (n)

Culture on L-J

Direct PCR (IS901)

â—†Samples for culture (n)

Replicates (n)

Culture positive (%)

Identification of isolates

Contaminated cultures (replicate %)

Species of birds

Samples for PCR (n)

Positive (%)

Species of birds

F1*

4

4

12

0

 

33.3%

 

4

0

 

F2*

29

29

87

S0

 

0%

 

8

0

 

F3*

12

12

36

0

 

5.6%

 

5

0

 

F4*

12

12

36

0

 

100%

 

11

1 (9.0)

Gallus domesticus

F5*

23

23

69

0

 

14.5%

 

16

0

 

F6*

4

4

12

2 (50)

M. terrae, M. engbaekii

66.7%

Gallus domesticus

4

0

 

F7*

16

16

48

2 (12.5)

Mycobacterium spp. (2 isolates)a

45.8%

Gallus domesticus

10

0

 

F8#

11

11

33

0

 

69.7%

 

11

0

 

F9#

11

11

33

0

 

57.6%

 

11

0

 

F10#

35

35

105

0

 

30.5%

 

35

0

 

F11#

43

43

129

0

 

23.3%

 

43

0

 

F12Ň‚

5

5

15

1 (20)

M. avium

20.0%

Pelecanus onocrotalus

2

0

 

F13Ň‚

12

12

36

0

 

11.1%

 

11

2 (18.2)

Ara spp. and Cacatua spp.

F14Ň‚

15

15

45

0

 

33.3%

 

11

2 (20)

Ara spp. and Anthracoceros malayanus

F15Ň‚

21

20

60

0

 

1.7%

 

21

1 (4.7)

Cacatua spp.

F16Ň‚

8

8

24

0

 

0%

 

0

0

 

F17Ň‚

3

2

6

0

 

33.3%

 

3

0

 

F18Ň‚

11

11

33

0

 

30.3%

 

11

0

 

F19Ň‚

25

23

69

1 (4,3)

Mycobacterium spp. (1)b

21.7%

Phigys solitaries

25

0

 

Total

300

296

888

6 (2.0)

 

248/888 (27.9%)

 

242

6 (2.5)

 
  1. *Village chickens, #Layer chickens and Ň‚captive exotic birds. No correlation was seen among the positive samples by culture and direct PCR. PCR was specific to M. avium (IS901). â—†Z-N stain of all concentrated avian fecal samples were negative. a sequencing was not performed for these isolates. b this isolate was positive for Z-N staining and PCR was not performed