Fig. 3

Co-transfection of shRNA-expressing plasmids targeting nucleoprotein gene. VerodogSLAM cells were first infected with CDV MOI 0.01 and then transfected with 1.2 μg of single or combinated pENTR/U6-Nis (0.4 μg each). Combination of the three N-specific shRNA plasmid vectors exhibited markedly reduced N transcript levels (a), infectious virus titers (b) and CPE (c). Average and standard deviations represent three independent transfections. Right vertical axis presents the percentage of virus inhibition highlighted on the markers (♦). More evident cytopathic effects are indicated by arrows. Values followed by the same lower case letters do not differ by Tukey’s test (p < 0.01). BLD, below limit of detection (20 TCID₅₀/mL). All figure panels (100X total magnification): representative data from at least three independent experiments using biological replicates