Table 2 Primers used for identification and amplification of MLST loci of M. bovis
Name | Sequence | Amplicon size (bp) |
|---|---|---|
16S rRNA forward | 5’-GAA TTC CGA GAG TTT GAT CCT GGC T-3’ | 1517 |
16S rRNA reverse | 5’-AAG CTT GAG GTA ATC CAT CCC CAC GTT C-3’ | |
uvrC forward | 5’-GAA TTC AAT GTG TCT ACT AGT CCT GG -3’ | 1620 |
uvrC reverse | 5’-AAG CTT AGC GTC ATA GAT TTT TGC ATA-3’ | |
adh-1 forward | 5’- GGA GTA ACT AGT TAC AAA GCA CTT A -3’ | 546 |
adh-1 reverse | 5’- TGC TAG TTG TTC AAA CAC GT -3’ | |
gltX forward | 5’- TGG TGA GTA TTC AAT AAG GT-3’ | 530 |
gltX reverse | 5’- GTT TTG AGA ATC ATT GCA − 3’ | |
gpsA forward | 5’- AAA ATG TGA GGA ATT GAT CA -3’ | 521 |
gpsA reverse | 5’- CCA ATT CCA ATT GCT AAA AC -3’ | |
gyrB forward | 5’- AGC TTG CTA ATT GCA CCA − 3’ | 678 |
gyrB reverse | 5’- TAT TTT GAA CAA ATT TTG CAT − 3’ | |
pta-2 forward | 5’- AAT TCG TAA TGG CAA AGA AG -3’ | 490 |
pta-2 reverse | 5’- CTT AGC TTT TCT TAC ATT TAG GT -3’ | |
tdk forward | 5’ –ATG TAT TTA AAA AGT GGA TTA GG -3’ | 572 |
tdk reverse | 5’- TAT CTC ATA GCT TTT TTA GC -3’ | |
tkt forward | 5’- CCA ACT TAT ATT ATG GTG CA -3’ | 533 |
tkt reverse | 5’- CCA CCA TAT AAA TTA ATG CC -3’ |