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Table 4 Primers used for amplification of each gene in this study

From: Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar

Target gene Primer name Primer sequences (5′ – 3′) PCR conditions Expected size (bp) References
For detection of pathogen
 TK TK-F ACG ATG ACT CCG ACT TTC 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min 647 Pang et al. [35]
TK-R CGT TGG AGG TAG GTG GTA
For sequence analysis
 gB gB-F CAA GGG CGG AAT TTG ATA GA 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min 440 This study
gB-R AAT GAG GCG ATG CCA GAT GC
 gG gG-F TTG TGC GCG TCT GTA TTA GG 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 30s); 72 °C 10 min 612 This study
gG-R CTC CAT AGG ACC GTC GAG TT
 gJ gJ-F GTT AAC GCC TCT CTG GAA CG 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min 667 This study
gJ-R TCG GGG AAG TAC CTG TAT CG
ICP4 fragment 1 ICP4a-F ACT GAT AGC TTT TCG TAC AGC ACG 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min 688 Chacon et al. [21]
ICP4a-R CAT CGG GAC ATT CTC CAG GTA GCA
ICP4 fragment 2 ICP4b-F CGA AAT CGG AAA AGC TTC AG 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min 624 This study
ICP4b-R CTC CAG CAA CAA CAC ATT GG