Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar

BMC Veterinary Research

Table 4 Primers used for amplification of each gene in this study

Target gene	Primer name	Primer sequences (5′ – 3′)	PCR conditions	Expected size (bp)	References
For detection of pathogen
TK	TK-F	ACG ATG ACT CCG ACT TTC	94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min	647	Pang et al. [35]
TK	TK-R	CGT TGG AGG TAG GTG GTA		647	Pang et al. [35]
For sequence analysis
gB	gB-F	CAA GGG CGG AAT TTG ATA GA	94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min	440	This study
gB	gB-R	AAT GAG GCG ATG CCA GAT GC		440	This study
gG	gG-F	TTG TGC GCG TCT GTA TTA GG	94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 30s); 72 °C 10 min	612	This study
gG	gG-R	CTC CAT AGG ACC GTC GAG TT		612	This study
gJ	gJ-F	GTT AAC GCC TCT CTG GAA CG	94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min	667	This study
gJ	gJ-R	TCG GGG AAG TAC CTG TAT CG		667	This study
ICP4 fragment 1	ICP4a-F	ACT GAT AGC TTT TCG TAC AGC ACG	94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min	688	Chacon et al. [21]
ICP4 fragment 1	ICP4a-R	CAT CGG GAC ATT CTC CAG GTA GCA		688	Chacon et al. [21]
ICP4 fragment 2	ICP4b-F	CGA AAT CGG AAA AGC TTC AG	94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min	624	This study
ICP4 fragment 2	ICP4b-R	CTC CAG CAA CAA CAC ATT GG		624	This study

ISSN: 1746-6148