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Table 4 Primers used for amplification of each gene in this study

From: Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar

Target gene

Primer name

Primer sequences (5′ – 3′)

PCR conditions

Expected size (bp)

References

For detection of pathogen

 TK

TK-F

ACG ATG ACT CCG ACT TTC

94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min

647

Pang et al. [35]

TK-R

CGT TGG AGG TAG GTG GTA

For sequence analysis

 gB

gB-F

CAA GGG CGG AAT TTG ATA GA

94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min

440

This study

gB-R

AAT GAG GCG ATG CCA GAT GC

 gG

gG-F

TTG TGC GCG TCT GTA TTA GG

94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 30s); 72 °C 10 min

612

This study

gG-R

CTC CAT AGG ACC GTC GAG TT

 gJ

gJ-F

GTT AAC GCC TCT CTG GAA CG

94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min

667

This study

gJ-R

TCG GGG AAG TAC CTG TAT CG

ICP4 fragment 1

ICP4a-F

ACT GAT AGC TTT TCG TAC AGC ACG

94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min

688

Chacon et al. [21]

ICP4a-R

CAT CGG GAC ATT CTC CAG GTA GCA

ICP4 fragment 2

ICP4b-F

CGA AAT CGG AAA AGC TTC AG

94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min

624

This study

ICP4b-R

CTC CAG CAA CAA CAC ATT GG