Target gene | Primer name | Primer sequences (5′ – 3′) | PCR conditions | Expected size (bp) | References |
---|---|---|---|---|---|
For detection of pathogen | |||||
TK | TK-F | ACG ATG ACT CCG ACT TTC | 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min | 647 | Pang et al. [35] |
TK-R | CGT TGG AGG TAG GTG GTA | ||||
For sequence analysis | |||||
gB | gB-F | CAA GGG CGG AAT TTG ATA GA | 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min | 440 | This study |
gB-R | AAT GAG GCG ATG CCA GAT GC | ||||
gG | gG-F | TTG TGC GCG TCT GTA TTA GG | 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 30s); 72 °C 10 min | 612 | This study |
gG-R | CTC CAT AGG ACC GTC GAG TT | ||||
gJ | gJ-F | GTT AAC GCC TCT CTG GAA CG | 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min | 667 | This study |
gJ-R | TCG GGG AAG TAC CTG TAT CG | ||||
ICP4 fragment 1 | ICP4a-F | ACT GAT AGC TTT TCG TAC AGC ACG | 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min | 688 | Chacon et al. [21] |
ICP4a-R | CAT CGG GAC ATT CTC CAG GTA GCA | ||||
ICP4 fragment 2 | ICP4b-F | CGA AAT CGG AAA AGC TTC AG | 94 °C 2 min; 35 × (94 °C 30s, 55 °C 30s, 72 °C 50s); 72 °C 10 min | 624 | This study |
ICP4b-R | CTC CAG CAA CAA CAC ATT GG |