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Table 5 The time-temperature profile for metagenomic analysis of the 16S rRNA V3-V4 hypervariable region

From: Insight into the intestinal microbiome of farrowing sows following the administration of garlic (Allium sativum) extract and probiotic bacteria cultures under farming conditions

NGS PCR profile

Step

Temperature

Time

No. of cycles

initial denaturation

98 °C

30 s

1

denaturation

98 °C

10 s

3–15

annealing

65 °C

75 s

 

final extension

65 °C

5 min

1

final hold

4–10 °C

∞

 
  1. Abbreviations: 16S rRNA 16S ribosomal RNA, NGS Next generation sequencing, 16S V3-V4 Regions of interest-specific primer
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BMC Veterinary Research

ISSN: 1746-6148

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