Fig. 1
Effect of AJSAF on H9–Ag-specific IgG and its isotype antibody titers in the immunized mice. Mice were s.c. injected with 0.2 ml of IH9V (107 TCID50/0.1 ml) alone or containing Quil A or AJSAF, or 0.2 ml of CH9V (107 TCID50/0.1 ml) on days 1 and 15. Sera were collected 14 days after the boosting immunization, and serum H9–Ag-specific IgG, IgG1, IgG2a, and IgG2b antibody titers were measured by ELISA. The values are presented as means ± SD (n = 6). Significant differences with IH9V alone group were designated as *P < 0.05, †P < 0.01, and ‡P < 0.001; those with CH9V group as §P < 0.05, #P < 0.01, and ¶P < 0.001