Table 4 Detection of clinical samples using the established duplex real-time PCR assay
Sample no. | Duplex TaqMan PCR assay (Ct value) | VetMAX™ ASFV Detection Kit (Ct value) | Sample no. | Duplex TaqMan PCR assay (Ct value) | VetMAX™ ASFV Detection Kit (Ct value) |
|---|---|---|---|---|---|
1 | 27.27/27.81 | 27.41/− | 14 | 34.19/34.64 | 35.74/− |
2 | 19.58/19.44 | 20.7/− | 15 | 31.05/29.86 | 31.98/− |
3 | 17.87/17.42 | 18.4/− | 16 | 33.45/33.85 | 33.9/− |
4 | 18.97/18.4 | 19.42/− | 17 | 32.91/31.9 | 32.77/− |
5 | 14.32/13.02 | 15.24/− | 18 | 31.97/31.84 | 32.99/− |
6 | 16.21/14.87 | 16.38/− | 19 | 30.97/30.12 | 31.94/− |
7 | 16.87/17.46 | 17.61/− | 20 | 34.49/33.73 | 36.52/− |
8 | 27.66/27.11 | 27.72/− | 21 | 33.99/35.61 | 35.64/− |
9 | 26.37/26.51 | 26.72/− | 22 | 35.62/37.0 | 35.19/− |
10 | 19.05/18.56 | 19.81/− | 23 | 34.51/35.51 | 35.42/− |
11 | 18.23/17.49 | 18.78/− | 24 | 37.16/39.76 | 36.99/− |
12 | 31.68/30.82 | 31.85/− | 25 | 36.12/38.4 | undetermined |
13 | 34.67/34.3 | 32.65/− | 26 | 36.95/− | undetermined |