Plasmid | Copy number | Inter-coefficient of variation | Intra-coefficient of variation | ||||
---|---|---|---|---|---|---|---|
Ct mean | Ct SD | CV% | Ct mean | Ct SD | CV% | ||
pUC57- B646L | 5.8 × 107 | 13.50 | 0.23 | 1.7 | 13.19 | 0.29 | 2.2 |
5.8 × 106 | 16.79 | 0.29 | 1.7 | 16.56 | 0.35 | 2.1 | |
5.8 × 105 | 19.53 | 0.12 | 0.6 | 19.57 | 0.03 | 0.1 | |
5.8 × 104 | 22.64 | 0.08 | 0.3 | 22.35 | 0.24 | 1.1 | |
5.8 × 103 | 26.02 | 0.14 | 0.5 | 25.99 | 0.12 | 0.5 | |
5.8 × 102 | 28.31 | 0.27 | 1 | 28.81 | 0.38 | 1.3 | |
5.8 × 101 | 31.75 | 0.08 | 0.3 | 32.05 | 0.27 | 0.8 | |
pUC57- MGF505-2R | 3.0 × 107 | 12.62 | 0.14 | 1.1 | 12.62 | 0.23 | 1.9 |
3.0 × 106 | 15.61 | 0.21 | 1.3 | 15.79 | 0.35 | 2.2 | |
3.0 × 105 | 19.02 | 0.08 | 0.4 | 18.94 | 0.38 | 2 | |
3.0 × 104 | 21.85 | 0.26 | 1.2 | 21.78 | 0.18 | 0.8 | |
3.0 × 103 | 25.82 | 0.22 | 0.9 | 25.60 | 0.37 | 1.5 | |
3.0 × 102 | 28.46 | 0.21 | 0.7 | 28.51 | 0.54 | 1.9 | |
3.0 × 101 | 31.43 | 0.16 | 0.5 | 31.70 | 0.76 | 2.4 |