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Table 3 Repeatability of duplex TaqMan real-time PCR

From: Development and evaluation of duplex TaqMan real-time PCR assay for detection and differentiation of wide-type and MGF505-2R gene-deleted African swine fever viruses

Plasmid

Copy number

Inter-coefficient of variation

Intra-coefficient of variation

Ct mean

Ct SD

CV%

Ct mean

Ct SD

CV%

pUC57-

B646L

5.8 × 107

13.50

0.23

1.7

13.19

0.29

2.2

5.8 × 106

16.79

0.29

1.7

16.56

0.35

2.1

5.8 × 105

19.53

0.12

0.6

19.57

0.03

0.1

5.8 × 104

22.64

0.08

0.3

22.35

0.24

1.1

5.8 × 103

26.02

0.14

0.5

25.99

0.12

0.5

5.8 × 102

28.31

0.27

1

28.81

0.38

1.3

5.8 × 101

31.75

0.08

0.3

32.05

0.27

0.8

pUC57-

MGF505-2R

3.0 × 107

12.62

0.14

1.1

12.62

0.23

1.9

3.0 × 106

15.61

0.21

1.3

15.79

0.35

2.2

3.0 × 105

19.02

0.08

0.4

18.94

0.38

2

3.0 × 104

21.85

0.26

1.2

21.78

0.18

0.8

3.0 × 103

25.82

0.22

0.9

25.60

0.37

1.5

3.0 × 102

28.46

0.21

0.7

28.51

0.54

1.9

3.0 × 101

31.43

0.16

0.5

31.70

0.76

2.4