Table 1 Primers and probes used for the duplex TaqMan Probe-based real-time RT-PCR
Primer/Probe | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|
B646L-F1 | GGGTGCATGTCATTCATCCT | 100 |
B646L-R1 | GTCATATCCGTTGCGAGGAA | |
B646L-P1 | FAM-AGGATGCTCCGATTCAGGGCACGTCCCA-BHQ1 | |
MGF505-2R-F1 | AGCTCTTGTTACTGTGGAAGG | 173 |
MGF505-2R-R1 | AAAACGTTCTAAAGCGTGGC | |
MGF505-2R-P1 | VIC-ACGCCGTCATAGGAGCCTTGCAGGGTGA-BHQ1 |