Fig. 1From: Development and evaluation of duplex TaqMan real-time PCR assay for detection and differentiation of wide-type and MGF505-2R gene-deleted African swine fever virusesSequence analysis and alignment of primers and probes in ASFVs. The nucleotides sequence of primers and probes are consistent among the genotype II reference ASFV strains. Compared with the genotype I reference strains, the forward primer and probe targeting B646L gene have one nucleotide mutation at A345G and T376G sites, respectively. The probe targeting MGF505-2R gene has one nucleotide mutation at T265C siteBack to article page