Fig. 4

Impact of WWOX on clonogenic survival and cell viability in malignant canine mast cell lines. a Canine C2 cells expressing scramble control or shRNA lentiviral constructs targeting WWOX (shWWOX-554 and shWWOX-1304) were treated with increasing doses of ionizing radiation and plated in 6-well dishes in methylcellulose media until formation of visible colonies. Cells were then fixed and stained with crystal violet and colonies greater than 50 cells were counted. After counting colonies, plating efficiency and survival fraction were calculated. Plating efficiency was defined as the number of colonies formed divided by the number of cells seeded in untreated (0 Gy) control groups. Surviving fraction was defined as the number of colonies formed divided by the number of cells seeded in irradiated groups, normalized to the plating efficiency. Experiments were repeated three times and average of results are shown. All experiments were performed in triplicate and data represent the mean +/− SD of three independent experiments (Statistical analysis: one-way ANOVA, *p ≤ 0.05)