Fig. 1

WWOX expression is reduced in canine MCTs and malignant mast cell lines. RNA was collected from (a) normal canine BMCMCs and fresh frozen canine MCT tissues and (b) malignant canine BR and C2 mast cell lines and quantitative RT-PCR for WWOX was performed. c Canine BR and C2 mast cell lines were left untreated or incubated with 100 ng/mL rcSCF for 24 h prior to collection. Protein lysates were generated, separated by SDS-PAGE and Western blotting for WWOX and β-actin was performed. d Fresh frozen canine MCT tissues and e malignant canine (BR and C2) and mouse (C57 and P815) mast cell lines were processed for protein lysates. Protein was separated by SDS-PAGE and Western blotting for WWOX and β-actin was performed. Full-length blots are presented in Supplementary Fig. 1. Three independent experiments were performed and all reactions were performed in triplicate. The experiments were repeated 3 times in the cell lines and twice for normal canine BMCMCs