Skip to main content

Table 1 List of predesigned assays and in-house designed primers and probes used for real time TaqMan qPCR

From: Determination of novel reference genes for improving gene expression data normalization in selected canine reproductive tissues – a multistudy analysis

Gene

Gene name

Accession number

Sequence

Amplicon length

COX2/PTGS2

cyclooxygenase-2

HQ_110882

Forward: 5′-GGA GCA TAA CAG AGT GTG TGA TGT G-3’

87 bp

   

Reverse: 5′-AAG TAT TAG CCT GCT CGT CTG GAA T-3’

 
   

Probe: 5′-CGC TCA TCA TCC CAT TCT GGG TGC T-3’

 

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

AB_028142

Forward: 5′-GCT GCC AAA TAT GAC GAC ATC A-3’

75 bp

   

Reverse: 5′-GTA GCC CAG GAT GCC TTT GAG-3′

 
   

Probe: 5′-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3’

 

IL-1b

interleukin 1b

NM_001037971

Forward: 5′-TGC CAA GAC CTG AAC CAC AGT-3′

97 bp

   

Reverse: 5′-CTG ACA CGA AAT GCC TCA GAC T-3′

 
   

Probe: 5′-CAT CCA GTT GCA AGT CTC CCA CCA GC-3′

 

LHR

luteinizing hormone receptor

XM_538486

Forward: 5′-TCA TCA TTT GTG CTT GCT ACA TTA AA-3’

98 bp

   

Reverse: 5′-CGC CAT TTT CTT AGC AAT CTT TG-3’

 
   

Probe: 5′-TGC AGT TCA AAA TCC AGA GCT GAT GGC-3’

 

MHCII

major histocompatibility complex class II

NM_001011723

Forward: 5′-GGA GAG CCC AAC ATC CTC ATC-3′

90 bp

   

Reverse: 5′-GGT GAC AGG GTT TCC ATT TCG-3′

 
   

Probe: 5′-TCG ACA AGT TCT CCC CAC C-3′

 

VEGFA

vascular endothelial growth factor A

NM_001003175

Forward: 5′-GTG CCC ACT GAG GAG TTC AAC-3’

72 bp

   

Reverse: 5′-CCC TAT GTG CTG GCC TTG AT-3’

 
   

Probe: 5′-CAC CAT GCA GAT TAT GCG GAT CAA ACC-3’

 

Gene

Gene name

Product no.

β-actin

actin beta

Cf03023880_g1

cyclophilin A/PPIA

cyclophilin A

Cf03986523_gH

EIF4H

eukaryotic translation initiation factor 4H

Cf02713640_m1

IGF2

insulin-like growth factor 2

Cf02647136_m1

KDELR2

endoplasmic reticulum lumen protein-retaining receptor 2

Cf02668050_m1

KDM4A

lysine-specific demethylase 4A

Cf02708629_m1

PTK2

focal adhesion kinase 1, protein tyrosine kinase 2

Cf02684608_m1