Table 1 Performance parameters of the real-time PCR assays
Assay | Slope | Y-Intercept | r2 | E (%) | Intra-assay CV (%) | Inter-assay CV (%) |
|---|---|---|---|---|---|---|
ISC-A | −3.3732 | 36.472 | 0.99 | 97.90 | ≤ 1.22 | ≤ 0.71 |
ISC-B | −3.3193 | 37.285 | 0.99 | 100.1 | ≤ 1.41 | ≤ 0.98 |
parA-A | −3.1270 | 38.134 | 0.98 | 108.8 | ≤ 0.81 | ≤ 1.37 |
parA-B | −3.3723 | 40.008 | 0.99 | 97.94 | ≤ 2.01 | ≤ 1.05 |
nahE | −3.4440 | 37.773 | 0.99 | 95.20 | ≤ 1.88 | ≤ 0.61 |