Enhancing the antibacterial activity of antimicrobial peptide PMAP-37(F34-R) by cholesterol modification

BMC Veterinary Research

Table 1 Amino acid sequences and biochemical parameters of PMAP-37, PMAP-37(F34-R), and Chol-37(F34-R)

Peptides	Sequence	Theoretical molecular weight	Measured molecular weight^a	Net charge^b	Theoretical pI^c	T_R (min)^d
PMAP-37	GLLSRLRDFLSDRGRRLGEKIERIGQKIKDLSEFFQS	4365.02	4364.96	+ 3	10.24	11.698
PMAP-37(F34-R)	GLLSRLRDFLSDRGRRLGEKIERIGQKIKDLSERFQS	4374.03	4374.07	+ 4	10.80	10.302
Chol-37(F34-R)	Chol-GLLSRLRDFLSDRGRRLGEKIERIGQKIKDLSERFQS	4786.67	4786.55	+ 3	-^e	11.705

^aMeasured Molecular weight (MW) was measured by MS
^bNet charge was calculated by antimicrobial peptide database. (http://aps.unmc.edu/AP/prediction/prediction_main.php)
^cpI was calculated by antimicrobial peptide analysis tool. (https://web.expasy.org/protparam/)
^dT_R, retention time measured by analytical HPLC
^e−, indicated the data not provided

ISSN: 1746-6148