Fig. 3

Expressions of ISGs induced by IFN-λ3 in primary PAMs. The primary PAMs were stimulated with different dose of IFN-λ3 12 h. qRT-PCR was performed to analyse the expression of OAS1(a), Mx1(b), IFITM3 (c), and ISG15 (d). The western blot was used to detect the proteins (e). The full-length blots are presented in Supplementary file 2 (a to d). The grey value of protein bands was measured by image J (f). Data were presented as mean ± SEM (N = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by Unpaired T-test