Fig. 1

Phylogenetic tree showing the relationship of the cat origin Mycoplasma sequences derived from the present study (marked with bold) and other Mycoplasma spp. isolated from different hosts in different countries (in brackets), based on analysis of a partial sequence of the 16S rRNA gene. GenBank accession numbers are shown, and Clostridium innocuum (GenBank: M23732) was used as the outgroup. Numbers at branches indicate bootstrap support levels (1,000 replicates). The evolutionary history was inferred by using the Maximum Likelihood method and Tamura-Nei model [27]. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed [28]. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [28]. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Tamura-Nei model, and then selecting the topology with superior log likelihood value. This analysis involved 29 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. There was a total of 538 positions in the final dataset. Evolutionary analyses were conducted in MEGA X [29]