Table 2 Results of direct microscopy, culture, and direct sample-PCR assays for the detection and identification of dermatophytes in 150 scales and hair samples
Direct microscopy | Dermatophyte culture | aPan-dermatophyte PCR | bOne-step PCR | Nested-PCR | Frequency (%) |
|---|---|---|---|---|---|
[No. positive = 111 (74%)] | [No. positive = 84 (56%)] | [No. positive = 87 (58%)] | [No. positive = 93 (62%)] | [No. positive = 108 (72%)] | |
+ | + | + | + | + | 51 (34) |
+ | + | – | – | – | 6 (4) |
+ | + | + | – | + | 9 (6) |
+ | + | – | – | + | 9 (6) |
+ | – | + | + | + | 6 (4) |
+ | – | – | – | – | 15 (10) |
+ | – | – | + | – | 6 (4) |
+ | – | + | – | + | 3 (2) |
+ | – | – | – | + | 6 (4) |
– | + | + | + | + | 9 (6) |
– | – | + | + | + | 6 (4) |
– | – | + | – | + | 3 (2) |
– | – | – | + | – | 15 (10) |
– | – | – | – | + | 6 (4) |