Fig. 7
a Western blot was used to assess the molecular characteristics of PK-resistant PrP material in the brains of TgBovXV mice. Samples from o-bTMEVV, bTME, and L-BSE inoculated mice were similar to each other and different from C-BSE. The antibody 6H4 anti-prion antibody was used for detection (1:10,000). b Glycosylation analysis of the western blot from panel A. The percentage of diglycosylated PrPSc comprised the majority of signal in the C-BSE sample. Likewise, the amount of diglycosylated PrPSc in o-bTMEAV was much higher compared to o-bTMEVV, bTME, and L-BSE. c Molecular weight analysis of the western blot from panel A. The molecular weight of the diglycosylated band of o-bTMEAV was less than other isolates