Fig. 5

Direct virucidal activity of baicalin on MDV. Compounds were mixed with MDV at 0 and 20 μg/mL respectively and incubated in a 37 °C incubator for 1.5 h, then inoculated onto CEF until harvest. The RNA of the cells were extracted for qRT-PCR to detect the expression level of the Meq gene and the gB gene (a). The virus quantity was detected by plaque counting (b). The indirect immunofluorescence assay showed the similar results with plaque counting (c). Data were expressed as mean ± SD from three independent experiments and analyzed by Student’s t-tests (**p < 0.01, *** p < 0.001)