Fig. 4

Effects of baicalin on MDV RB-1B replication are dependent on the time of baicalin application. a The gene expression level of Meq gene and gB gene were detected by qRT-PCR b Viral quantity was detected by plaque counting. 0 μg/mL indicates no baicalin treatment, P1 indicates pretreatment with 20 μg/mL baicalin for 2 h before virus adsorption, P2 indicates 20 μg/mL baicalin and the virus were added at the same time, and P3 indicates 20 μg/mL baicalin was added after virus adsorption. (C + D) 20 μg/mL baicalin was added at 0, 1, 6, 12, and 24 h after virus adsorption, and the virus was harvested 96 h post infection for detection of Meq and gB expression by qRT-PCR (c), and virus quantification by plaque counting (d). Data were expressed as mean ± SD from three independent experiments and analyzed by Student’s t-tests (*p < 0.05,**p < 0.01, *** p < 0.001)