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Table 1 Metabolites differences between captive and wild North China leopard achieved from the analyses of feces samples submitted to liquid chromatography/mass spectrometer (LC-MS) analyses using an Ultra High Performance Liquid Chromatography (UHPLC) system

From: Gut microbiota and fecal metabolites in captive and wild North China leopard (Panthera pardus japonensis) by comparsion using 16 s rRNA gene sequencing and LC/MS-based metabolomics

No. Metabolites P value VIP Regulated
999 N-acetyl-L-alanine 0. 006 1. 602 Upb
1155 anthranilic acid (vitamin L1) 0. 004 1. 723 Downb
1331 hydroxyhydroquinone 0. 022 1. 595 Upa
1474 trans-cinnamate 0. 044 1. 534 Upa
1492 L-methionine 0. 049 1. 514 Upa
1814 isovalerylglycine 0. 041 1. 517 Upa
1945 5-hydroxymethyluracil 0. 022 1. 529 Downa
2713 tiopronin 0. 044 1. 610 Downa
2850 kynurenic acid 0. 011 1. 630 Upa
2856 3-indolepropionic acid 0. 011 1. 640 Upa
2869 glycyl-L-leucine 0. 039 1. 571 Upa
2925 N-acetyl-DL-methionine 0. 019 1. 617 Upa
3103 1, 3-dimethyluric acid 0. 025 1. 659 Upa
4329 N-acetyl-L-tyrosine 0. 018 1. 641 Upa
4374 D-quinovose 0. 004 1. 603 Upb
5127 L-gulonic gamma-lactone 0. 024 1. 521 Upa
5361 tetrahydrobiopterin 0. 037 1. 608 Downa
5372 pentadecanoic Acid 0. 005 1. 599 Downb
5531 dioxybenzone 0. 045 1. 417 Upa
5714 quadrone 0. 000 1. 725 Downb
6094 5-hydroxymethylcytidine 0. 019 1. 619 Upa
7596 oleic acid 0. 032 1. 582 Downa
7742 stearic acid 0. 034 1. 542 Downa
10198 beta-Estradiol 0. 007 1. 687 Downb
10469 salidroside 0. 039 1. 582 Upa
10588 eicosapentaenoic Acid 0. 049 1. 501 Downa
11683 L-iditol 0. 044 1. 522 Downa
13489 ethynodiol diacetate 0. 016 1. 637 Upa
13779 tamsulosin 0. 000 1. 707 Downb
18005 muramic acid 0. 000 1. 689 Downb
24886 deoxycholic acid 0. 006 1. 705 Upb
  1. Note: ameans significant difference, and bmeans extremely significant difference, Up means the fecal metabolites of captive North China leopard are higher than those in the wild, Down means the fecal metabolites of wild North China leopard are higher than those in the captive.