TY - JOUR AU - Orłowska, Blanka AU - Krajewska-Wędzina, Monika AU - Augustynowicz-Kopeć, Ewa AU - Kozińska, Monika AU - Brzezińska, Sylwia AU - Zabost, Anna AU - Didkowska, Anna AU - Welz, Mirosław AU - Kaczor, Stanisław AU - Żmuda, Piotr AU - Anusz, Krzysztof PY - 2020 DA - 2020/09/29 TI - Epidemiological characterization of Mycobacterium caprae strains isolated from wildlife in the Bieszczady Mountains, on the border of Southeast Poland JO - BMC Veterinary Research SP - 362 VL - 16 IS - 1 AB - The majority of animal tuberculosis (TB) cases reported in wildlife in Poland over the past 20 years have concerned the European bison inhabiting the Bieszczady Mountains in Southeast Poland: an area running along the border of Southeast Poland. As no TB cases have been reported in domestic animals in this region since 2005, any occurrence of TB in the free-living animals inhabiting this area might pose a real threat to local livestock and result in the loss of disease-free status. The aim of the study was to describe the occurrence of tuberculosis in the wildlife of the Bieszczady Mountains and determine the microbiological and molecular characteristics of any cultured strains. Lymph node samples were collected for analysis from 274 free-living animals, including European bison, red foxes, badgers, red deer, wild boar and roe deer between 2011 and 2017. Löwenstein–Jensen and Stonebrink media were used for culture. Molecular identification of strains was performed based on hsp65 sequence analysis, the GenoType®MTBC (Hain Lifescience, Germany) test, spoligotyping and MIRU-VNTR analysis. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-020-02581-3 DO - 10.1186/s12917-020-02581-3 ID - Orłowska2020 ER -