The pathogenesis of a North American H5N2 clade 2.3.4.4 group A highly pathogenic avian influenza virus in surf scoters (Melanitta perspicillata)

Table 1 Serostatus of surf scoters pre- and post-inoculation with H5 HPAIV NP/WA/14 (H5N2). Whole blood was collected 14 dpi and serum was separated for antibody analysis

Bird age/sex^a	Pre-challenge ELISA^b	Post-challenge ELISA^b	Log₂ serum HAI titer
2/M	neg	pos	7
4/M	neg	pos	6
4/M	neg	pos	8
5/M	neg	pos	9
6/M	neg	pos	5
6/M	neg	pos	7
7/M	neg	pos	7
16/M	neg	nd	nd
16/F	neg	nd	nd

Anti-influenza A nucleoprotein IgY response was determined by blocking ELISA using commercial kit. Serum hemagglutinin inhibition titers were determined according to the standard USDA HI procedure (Pedersen et al. 2014)
^aAge reported in years. M = male, F = female
^bneg = seronegative for anti-influenza A NP IgY serum antibodies
Pos Seropositive for anti-influenza A NP IgY serum antibodies, Nd Not done, the birds had died

ISSN: 1746-6148