Fig. 6

Scheme of the main effects of the treatment with doxorubicin (DOXO) and canine amniotic membrane cells (AMCs) in IPC-366 cells. The proliferation and angiogenesis of IPC-366 cells were increased due to the influence of PCNA, TGF-β1 and VEGF; however, treatment with DOXO + AMC association reversed this situation. Moreover, the treatment with the DOXO + AMC decreased IL-10 levels, which may have allowed tumour cells to be recognised by the components of the immune system and to decrease the inflammatory process. In addition, after treatment with DOXO + AMC there was an increase of cells in the G1/G0 phase and decrease in those in the G2/M phase due to a reduction in cell duplication. Furthermore, after treatment with the combination, there were reductions in the levels of P5, DHEA and DHT, while there were increases in E2 and SO4E, important steroid hormones in the steroidigenic pathway. We also observed that there was an increase in ER expression, which can be used as a therapeutic target

P4: progesterone; A4: androstenedione; E1: estrone