Fig. 1

Heat resistance curve. Note: Diluted virus solution was aliquoted into 8-strip PCR tubes at 50 µL per tube. The heat treatment temperature and period were set using a PCR machine. The processing treatment temperature range was 40 °C to 80 °C, with a total of 23 temperature gradients. The heat treatment time was between 0 s and 1200s, with a total of 23 gradients. All test samples were analyzed in triplicate for each temperature and time point. After the heat treatment, 50 µL of DMEM was added to each tube. After mixing, the contents of each tube were inoculated into a 96-well plate with a monolayer of PK-15 cells. The cytopathic conditions were observed and recorded 42 h after virus inoculation. The time point was set as the time of 100% inactivation of the virus at the given temperature without any cytopathic effect (CPE)