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Table 1 A vaccine sample concentration (ng/ml) obtained from calibration curve

From: Development of a double-recombinant antibody sandwich ELISA for quantitative detection of epsilon toxoid concentration in inactivated Clostridium perfringens vaccines

Optical density (450 nm) Vaccine sample conc (ng/ml)
Std Conc (ng/ml) Values Mean SD CV% Values Mean SD CV%
5 0.413
0.352
0.3825 0.043134 11.31 2039
2350
2194 219 10
50 0.441
0.426
0.4335 0.010607 2.44     
500 0.638
0.635
0.6365 0.002121 0.31     
5000 1.459
1.471
1.465 0.008485 0.54     
50,000 1.806
1.699
1.7525 0.07566 4.28     
  1. Standard curve was based on five serial dilutions of ETX with a concentration range of 5 to 50,000 ng/ml.
  2. CV%: Coefficient of variation expressed as percentage; Std conc Standard concentration; SD Standard deviation.