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Table 1 A vaccine sample concentration (ng/ml) obtained from calibration curve

From: Development of a double-recombinant antibody sandwich ELISA for quantitative detection of epsilon toxoid concentration in inactivated Clostridium perfringens vaccines

Optical density (450 nm)

Vaccine sample conc (ng/ml)

Std Conc (ng/ml)

Values

Mean

SD

CV%

Values

Mean

SD

CV%

5

0.413

0.352

0.3825

0.043134

11.31

2039

2350

2194

219

10

50

0.441

0.426

0.4335

0.010607

2.44

    

500

0.638

0.635

0.6365

0.002121

0.31

    

5000

1.459

1.471

1.465

0.008485

0.54

    

50,000

1.806

1.699

1.7525

0.07566

4.28

    
  1. Standard curve was based on five serial dilutions of ETX with a concentration range of 5 to 50,000 ng/ml.
  2. CV%: Coefficient of variation expressed as percentage; Std conc Standard concentration; SD Standard deviation.
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BMC Veterinary Research

ISSN: 1746-6148

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