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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of a double-recombinant antibody sandwich ELISA for quantitative detection of epsilon toxoid concentration in inactivated Clostridium perfringens vaccines

Fig. 2

Schematic illustrations of double-recombinant antibody sandwich ELISA for quantitative measurement of ETX. (1) Well is coated with the B1 phage VH antibody isolated from the DAb library and the uncoated surface is blocked by 3% BSA/PBS. (2) The C. perfringens epsilon toxoid binds to the coated B1 phage VH antibody. (3) G2 soluble scFv antibody selected from Tomlinson I + J libraries as the detector antibody binds to epsilon toxoid. (4) HRP-conjugated monoclonal anti-Polyhistidine antibody as the conjugate antibody binds to hexahistidine tag fused to G2 scFv

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BMC Veterinary Research

ISSN: 1746-6148

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