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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Effects of transport stress on pathological injury and expression of main heat shock proteins in the caprine stomach

Fig. 4

The effects of transportation-induced transport stress on abomasum of goats. a Hematoxylin and eosin stained-abomasum sections from control and transportation-induced transport stress goats. Scale bar 100 μm. b Immunohistochemical staining showing the expression of HSP27, HSP70 and HSP90 in abomasum of goats from control group and transported groups. NC, negative control. Scale bar 50 μm. c Western blot of the HSP27, HSP70 and HSP90 proteins in the abomasum of 2 h and 6 h transportation-treated goats. β-Actin was used as loading control. d Quantitative analysis of western blot using Image Pro Plus 6.0. e Real-time PCR analysis of the HSP27, HSP70 and HSP90 mRNA levels in the abomasum of 2 h and 6 h transportation-treated goats. f ELISA analysis of the HSP27, HSP70 and HSP90 concentrations in the abomasum of 2 h and 6 h transportation-treated goats. Data are presented as the means ± SD; *p < 0.05

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BMC Veterinary Research

ISSN: 1746-6148

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