Table 3 Primer sequence of selected genes used in rtPCR analysis
Gene | Primer sequence 5’-3’ | Gene bank accession number | Annealing temperature | Reference |
|---|---|---|---|---|
GAPDH | 5'-CAGCAATGCATCCTGCAC-3' 5'-GAGTTGCTGTTGAAGTCACAGG-3' | XM_017592435.1 | 60ºC | Nakahara et al. [44] |
VEGF | 5'-AGGCTGCACCCACGACAGAA-3' 5'-CTTTGGTCTGCATTCACATC-3' | NM_001110333.2 | 55ºC | Hu et al. [45] |
bFGF | 5'-GTCAAACTACAGCTCCAAGC-3' 5'-TTTATACTGCCCAGTTCGTT-3' | NM_019305.2 | 50 ºC | Zhu et al. [46] |
αSMA | 5’-CGATAGAACACGGCATCATC-3’ 5’-CATCAGGCAGTTCGTAGCTC-3’ | NM_031004.2 | 58 ºC | Reza Ghassemifar et al. [32] |