TY - JOUR AU - Elbialy, Zizy I. AU - Atiba, Ayman AU - Abdelnaby, Aml AU - Al-Hawary, Ibrahim I. AU - Elsheshtawy, Ahmed AU - El-Serehy, Hamed A. AU - Abdel-Daim, Mohamed M. AU - Fadl, Sabreen E. AU - Assar, Doaa H. PY - 2020 DA - 2020/09/24 TI - Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression JO - BMC Veterinary Research SP - 352 VL - 16 IS - 1 AB - Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR. SN - 1746-6148 UR - https://doi.org/10.1186/s12917-020-02566-2 DO - 10.1186/s12917-020-02566-2 ID - Elbialy2020 ER -